biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:326-330, 2006 | DOI: 10.1007/s10535-006-0045-x

Efficient regeneration system from wheat leaf base segments

K. Haliloglu1
1 Faculty of Agriculture, Department of Field Crops, Ataturk University, Erzurum, Turkey

Efficient plant regeneration system from leaf base segments of wheat (Triticum aestivum L.) was developed. The factors affecting the callus formation and regeneration capacity of leaf segments of two genotypes; Bobwhite and Pavon 76, were investigated. The highest number of somatic embryos (SE) was obtained on Murashige and Skoog medium supplemented with 2 mg dm-3 2,4-dichlorophenoxyacetic acid + 1 mg dm-3 naphthalenacetic acid (14.7 SE per segment). Highest frequency of embryogenic callus (96 %) and somatic embryo formation (24.3 SE per segment) were achieved in the first segments. The highest plantlet regeneration was obtained after transfer of embryogenic calli to regeneration medium supplemented with 1 mg dm-3 kinetin (6.3 plantlets per segment).

Keywords: auxins; cytokinins; somatic embryos; Triticum aestivum L.
Subjects: auxins; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, micropropagation, propagation; Triticum aestivum; wheat

Received: October 12, 2004; Accepted: April 21, 2005; Published: September 1, 2006  Show citation

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Haliloglu, K. (2006). Efficient regeneration system from wheat leaf base segments. Biologia plantarum, 50(3), 326-330. doi: 10.1007/s10535-006-0045-x
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